Combined hydrogen peroxide and nitric oxide priming modulate salt stress tolerance in acclimated and non-acclimated oilseed rape (Brassica napus L.) plants

Document Type : Research Paper


1 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran.

2 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.

3 Department of Biology, Payame Noor University (PNU), Tehran, Iran.


We examined the combined effects of hydrogen peroxide (H2O2) and nitric oxide (NO) on the responses of oilseed rape(Brassica napus L.) plants to salt stress under acclimated and non-acclimated conditions. The results of the shoot and root dry weight traits together with the measurement of malondialdehyde (MDA) indicated that salt acclimation with a low concentration of NaCl (50 mM) could not alleviate the inhibitory effect of high salinity (200 mM NaCl). Under acclimated conditions, seed priming with H2O2 or NO resulted in effective protection against salt stress, however, maximum amelioration of salt stress was found by the combined treatments of H2O2 + NO. Interestingly, in the salt-exposed non-acclimated plants, only seed priming with H2O2 + NO was effective in improving salt tolerance. Pretreatment with H2O2 + NO tended to limit Na translocation into photosynthetic organs to prevent salt damages. Additionally, a large increase in salicylic acid contentwas correlated with phenylalanine ammonia lyase activation and flavonoid biosynthesis was observed when oilseed rape plants exposed to salinity in the presence of H2O2+NO. Interestingly, in this study, endogenous NO content of H2O2–primed plants exhibited a significant increase under non-saline conditions, indicating that H2O2 influences NO accumulation. In addition, oilseed rape plants primed with H2O2 + NO exhibited lower MDA and H2O2 content, contributing to the better induction of antioxidative enzyme activities. Higher levels of antioxidant enzyme activities maintained the integrity of cell membranes, resulting in better plant growth under salt stress. Taken together, our results revealed that oilseed rape plants pretreated with H2O2 + NO exhibited more effective tolerance to salt stress than plants that were pretreated with H2O2 or NO alone.


Article Title [فارسی]

بهبود تحمل به شوری در گیاه کلزا توسط پرایمینگ ترکیبی پراکسید هیدروژن و اکسید نیتریک در شرایط عادت دهی و بدون عادت دهی به شوری

Authors [فارسی]

  • زهرا کریمی 1
  • جلیل خارا 2
  • قادر حبیبی 3
1 گروه زیست شناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه.
2 گروه زیست شناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه.
3 گروه زیست شناسی، دانشگاه پیام نور، تهران.
Abstract [فارسی]

در این پژوهش، تأثیر پرایمینگ بذور با پراکسید هیدروژن (H2O2)، اکسید نیتریک (NO) یا ترکیب H2O2+NO بر پاسخ های گیاه کلزا به شوری در شرایط عادت­ دهی و  بدون عادت­ دهی به شوری مورد مطالعه قرار گرفت. نتایج حاصل از سنجش صفات وزن خشک ساقه و ریشه به همراه سنجش مالون دی آلدئید (MDA) نشان دادند که کاربرد غلظت اندک کلرید سدیم (50 میلی مولار) به عنوان عامل عادت­ دهی به شوری، نتوانست باعث تخفیف اثرات زیانبار شوری بالا (200 میلی مولار) شود. در عین حال، در شرایط عادت­ دهی، پرایمینگ بذور با H2O2یا NO باعث تخفیف اثرات تنش شوری بر رشد کلزا شد، هرچند بیشترین تخفیف توسط ترکیب H2O2+NO به دست آمد. جالب توجه این که در گیاهان بدون عادت­ دهی به شوری، تنها پرایمینگ ترکیبی بذور با H2O2+NO در بهبود تحمل به شوری موثر بود. این تخفیف تنش توسط پرایمینگ ترکیبی در شرایط شوری، با کاهش انتقال سدیم به اندام هوایی، افزایش مقدار اسید سالیسیلیک، فلاوونوئیدها و فعالیت آنزیم فنیل آلانین آمونیالیاز همراه بود. پرایمینگ بذور با پراکسید هیدروژن در شرایط غیر­شور، باعث افزایش مقدار NO داخلی شد. همچنین، پرایمینگ بذور با ترکیب H2O2+NO از طریق کاهش MDA و H2O2داخلی وافزایش فعالیت آنزیم­ های سیستم آنتی اکسیدان، باعث حفظ تمامیت غشاها و در نتیجه بهبود رشد در شرایط شوری گردید. نتایج این تحقیق نشان داد که پرایمینگ ترکیبی بهتر از پرایمینگ تنها توانست اثرات زیانبار شوری در گیاه کلزا را تخفیف دهد.

Keywords [فارسی]

  • اسید سالیسیلیک
  • اکسید نیتریک
  • پرایمینگ ترکیبی
  • شوری
  • فلاوونوئید
  • کلزا
  • هومئوستازی یونی
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