Expression analysis and physiological response of sunTIP7 aquaporin gene to different water regimes in sunflower

Document Type : Research Paper

Authors

1 Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

2 Instituto de Recursos Naturales y Agrobiologia (IRNAS), Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.

Abstract

The response of a plant to drought stress is positively related to physiological traits and gene expression. Various recent studies suggest that membrane channel proteins, named aquaporin (AQPs), by affecting stomatal conductance behavior could be involved in plant responses to stress conditions. The sunTIP7 gene is a member of AQPs protein that is included in different environmental stress such as drought stress. In this study in order to investigate the sunTIP7 gene expression and its relation with relative water content (RWC), stomatal conductance, shoot fresh and dry weight, root area, chlorophyll index (SPAD) and electrolyte leakage (EL), six selected sunflower (Helianthus annuus L.) inbred lines were planted in a greenhouse under normal irrigation and water deficit conditions. The water deficit treatments were applied during 4th leaflet to flowering stage. Drought conditions reduced crop growth and physiological traits. The highest stomatal conductance was found in the C138 line under both conditions. Sunflower lines had different fold change expression of sunTIP7 gene under drought stress. The expression of the sunTIP7 genewas the lowest in C138, and this downregulation may explain its highest stomatal conductance. However, there was not any clear relationship between physiological traits and expression of sunTIP7 gene in all six sunflower inbred lines. These results suggest that drought tolerance in sunflower is a complex trait and there is no simple molecular explanation for drought tolerance in sunflower lines.

Keywords


Article Title [فارسی]

تجزیه بیان و پاسخ فیزیولوژیک ژن آکواپورین sunTIP7 تحت رژیم های مختلف آبیاری در آفتابگردان

Abstract [فارسی]

پاسخ گیاه به تنش خشکی با صفات فیزیولوژیکی و بیان ژن رابطه مثبتی دارد. مطالعات متعدد اخیر نشان می­دهد که پروتئین­های کانال غشایی به نام  Aquaporin (AQPs) با تأثیر بر رفتار هدایت روزنه می­ توانند در پاسخ گیاهان به شرایط تنش نقش داشته باشند. ژنsunTIP7  عضو خانواده AQPs  می ­باشد  که در تنش­ های مختلف محیطی از جمله تنش خشکی درگیر می ­باشد. در این مطالعه به منظور بررسی بیان ژن sunTIP7 و ارتباط آن با صفات محتوای آب نسبی (RWC)، هدایت روزنه ­ا­ی، وزن تر و خشک بخش هوایی گیاه، سطح ریشه، شاخص کلروفیل (SPAD) و نشت الکترولیتی در شرایط تنش خشکی، شش لاین اینبرد انتخابی آفتابگردان (Helianthus annuus L.) در شرایط آبیاری نرمال و کم ­آبیاری در گلخانه کشت شدند. تیمار کم ­آبیاری در مرحله چهار برگی تا زمان گلدهی اعمال گردید و موجب کاهش صفات فیزیولوژیک و رشدی گیاه شد. بیشترین میزان هدایت روزنه­ ا­ی در هر دو شرایط در لاین C138 مشاهده شد. مقدار بیان ژن sunTIP7  در لاین­ های آفتابگردان در شرایط تنش متفاوت بود.  کمترین میزان بیان این ژن در لاین C138 مشاهده شد که این کاهش بیان احتمالا با هدایت روزنه­­ ای بالا در این لاین مرتبط است. با این حال رابطه مشخصی بین بیان صفات فیزیولوژیک و بیان ژن sunTIP7 در لاین­ های مورد بررسی مشاهده نشد. این نتایج نشان می­ دهد که تحمل به خشکی در آفتابگردان یک صفت پیچیده بوده و یک راهکار مولکولی ساده قادر به توضیح تحمل خشکی در لاین­ های اینبرد آفتابگردان نیست.

Keywords [فارسی]

  • آفتابگردان
  • آکواپورین
  • تنش خشکی
  • هدایت روزنه ای
  • sunTIP7
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