Karyotype analysis of some Allium species in Iran

Document Type : Research Paper

Authors

1 Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran.

2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran.

Abstract

The karyotypes of 10 species (15 accessions) of Allium from Iran were investigated using the squash technique and 1% (w/v) aceto-iron-hematoxylin stain. The basic chromosome number was x = 8, and only in A. giganteum (1) x = 7. Karyotypes of 14 taxa of Allium were diploid with 2n = 16; only A. macrochaetum was tetraploid with 2n = 32. Satellite chromosomes were seen in A. asarense. All karyotypes were symmetrical, consisting of metacentric and submetacentric chromosome pairs. Only A. caspium and A. stipitatum (1) had subtelocentric chromosomes. Karyotype analysis according to Stebbins categories placed the studied taxa in symmetric classes of 1A and 2A, indicating a symmetric karyotype. The results of the analysis of variance showed significant differences for total chromosome length (TCL), mean chromosome length (CL), long arm length (LA), short arm length (SA) and intrachromosomal asymmetry index (A1). The longest chromosome length was detected on A. asarense, A. elburzense, A. giganteum (3), A. rotundum and A. stipitatum (3) (17.9-19.7 μm), while A. ampeloperasum demonstrated the shortest value (8.2 μm). Results of cluster analysis based on chromosomal parameters classified the taxa into four groups using the unweighted pair group method with arithmetic mean (UPGMA). Using principal component analysis, the first three components determined 97.3% of the total variation. The grouping of the taxa based on the 2-D scatter plot using the first two principal components, corresponding to the results of the karyotypic characteristics.

Keywords

References

Akhavan A, Saeidi H, Zarr Sh and Rahiminejad MR, 2015. Chromosome numbers and karyotype features of selected species of Allium L. (Amaryllidaceae) sect. Acanthoprasonin Iran. The Iranian Journal of Botany 21(2): 158-164.
Ao C, 2008. Chromosome numbers and karyotypes of Allium przewalskianum populations. Acta Biologica Cracoviensia Series Botanica 50(1): 43-49.
Aryakia E, Karimi HR, Naghavi MR and Shahzadeh Fazeli SA, 2016. Morphological characterization of intra- and interspecific diversity in some Iranian wild Allium species. Euphytica 211(2): 185-200.
Battaglia E, 1957. Allium ascalonicum L., A. fistulosum L., A. cepa L.: Analisi Cariotipica. Caryologia 10(1): 1-28.
Choi HJ and Oh BU, 2011. A partial revision of Allium (Amaryllidaceae) in Korea and north-eastern China. Botanical Journal of the Linnean Society 167: 153-211.
Darlington CD and Haque A, 1955. The timing of mitosis and meiosis in Allium ascalonicum: a problem of differentiation. Heredity 9: 117-127.
De Sarker D, Johnson MAT, Reynolds A and Brandham PE, 1997. Cytology of the highly polyploid disjunct species, Allium dregeanum (Alliaceae), and of some Eurasian relatives. Botanical Journal of the Linnean Society 124(4): 361-373.
Dolatyari A, Saeidi Mehrvarz Sh, Shahzadeh Fazeli SA, Naghavi MR and Fritsch RM, 2018. Karyological studies of Iranian Allium L. (Amaryllidaceae) species with focus on sect. Acanthoprason. 1. Mitotic chromosomes. Plant Systematics and Evolution 304: 583-606.
Ebrahimi R, Zamani Z and Kashi A, 2009. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Scientia Horticulturae 119(4): 345-351.
Fritsch RM, 2018. Allium monophyllum (Amaryllidaceae) is a diploid species. Phytotaxa 333(2): 298-300.
Fritsch RM and Abbasi M, 2013. A Taxonomic Review of Allium subg. Melanocrommyum in Iran. IPK Gatersleben, Germany.
Fritsch RM and Astanova SB, 1998. Uniform karyotypes in different sections of Allium L. subgen. Melanocrommyum (Webb & Berth.) Rouy from Central Asia. Feddes Repertorium 109(7-8): 539-549.
 Fritsch RM, Blattner FR and Gurushidze M, 2010. New classification of Allium L. subg. Melanocrommyum (Webb & Berrth) Rouy (Alliaceae) based on molecular and morphological characters. Phyton 49: 145-220.
Fritsch RM and Friesen N, 2002. Evolution, domestication and taxonomy. In: Rabinowitch HD and Currah L (eds.). Allium Crop Science. Pp. 5-30. CABI Publishing, Wallingford, UK.
Fritsch RM and Maroofi H, 2010. New species and new records of Allium L. (Alliaceae)
from Iran. Phyton 50(1): 1-26.
Fritsch RM, Matin F and Klaas M, 2001. Allium vavilovii M. Popov et Vved. and a new Iranian species are the closest among the known relatives of the common onion A. cepa L. (Alliaceae). Genetic Resources and Crop Evolution 48: 401-408.
Ghaffari SM, 2006. New or rare chromosome counts of some angiosperm species from Iran. The Iranian Journal of Botany 11(2): 185-192.
Ghodrati Azadi H, Ghaffari SM, Riazi GH, Ahmadian Sh and Vahedi F, 2008. Antiproliferative activity of chloroformic extract of Persian shallot, Allium hirtifolium, on tumor cell lines. Cytotechnology 56(3): 179-185.
Guetat A, Rosato M, Rossello JA and Boussaid M, 2015. Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situ hybridization of rDNA sites and conventional stainings. Turkish Journal of Botany 39(5): 796-807.
Gurushidze M, Fuchs J and Blattner FR, 2012. The evolution of genome size variation in drumstick onions (Allium subgenus Melanocrommyum). Systematic Botany 37(1): 96-104.
Han T-S, Zheng Q-J, Onstein RE, Rojas‐Andrés BM, Hauenschild F, Muellner‐Riehl AN and Xing Y-W, 2020. Polyploidy promotes species diversification of Allium through ecological shifts. New Phytologist 225(1): 571-583.
Havey RM, 2002. Genome organization in Allium. In Rabinowitch HD and Currah L (eds.). Allium Crop Science: Recent Advances. Pp. 5-30. CABI Publ., UK.
Hosseini Sh, 2018. Karyological studies of some Allium L. (Amaryllidaceae) species in Iran. The Iranian Journal of Botany 24(1): 65-71.
Hosseini Sh and Go R, 2010. Cytogenetic study of some Allium species (subgenus Allium and Melanocrommyum) in Iran. Cytologia 75(1): 99-108.
Khorasani M, Saeidi Mehrvarz Sh and Zarre Sh, 2018. The genus Allium (Amaryllidaceae) in Iran: on the status of Allium ampeloprasum L. and its relatives. Nova Biologica Reperta 5(3): 299-306.
Konvicka O and Levan A, 1972. Chromosome studies in Allium sativum. Hereditas 72: 129-148.
Kuzmanov B, 1993. Chromosome numbers of Bulgarian angiosperrns: an introduction to a chromosome atlas of the Bulgarian flora. Flora Mediterranea 3: 19-163.
Kyung KH, 2012. Antimicrobial properties of Allium species. Current Opinion in Biotechnology 23(2): 142-147.
Levan A, Fredga K and Sandberg AA, 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52(2): 201-220.
Love A, 1976. IOPB chromosome number reports LII. Taxon 25(2-3): 341-346.
Miri SM and Roughani A, 2018. Allium species growing in Iran: chemical compositions and pharmacological activity. Proceedings of the First National Congress and International Fair of Medicinal Plants and Strategies for Persian Medicine that Affect Diabetes, October 9-11, Mashhad, Iran.
Miri SM and Roughani A, 2019. Allium species growing in Iran: botany and distribution. Proceedings of the 2nd International Conference on Medicinal Plants, Organic Farming, Natural and Pharmaceutical Ingredients, February 13-14, Mashhad, Iran.
Miryeganeh M and Movafeghi A, 2011. Karyotype analysis in some species of Allium section Allium (Alliaceae). Romanian Journal of Biology - Plant Biology 56(1): 17-27.
Mousavi A, Kashi A, Davoodi D and Sanei Shariatpanahi M, 2006. Characterization of an Allium cultivated in Iran: the Persian leek. Belgian Journal of Botany 139(1): 115-123.
Murray BG, 2005. When does intraspecific C-value variation become taxonomically significant? Annals of Botany 95: 119-125.
Olla LV and DeFilipps RA, 1969. A study of the great-headed garlic in Illinois. Illinois State Academy of Science Transactions 62(4): 402-409.
Özhatay N and Johnson MAT, 1996. Some karyological remarks on Turkish Allium sect. Allium, Bellevalia, Muscari, and Ornithogalum subg. Ornithogalum. Bocconea 5: 239-249.
Paknia R and Karimzadeh G, 2011. Karyotypic study and chromosome evolution in some Iranian local onion populations. Journal of Plant Physiology and Breeding. 1(1): 49-62.
Panahandeh J and Mahna N, 2011. The karyomorphology of Allium hirtifolium Bioss., a less known edible species from Iran. Journal of Plant Physiology and Breeding 1(2): 53-57.
Paszko B, 2006. A critical review and a new proposal of karyotype asymmetry indices. Plant Systematics and Evolution 258: 39-48.
Peruzzi L and Eroğlu HE, 2013. Karyotype asymmetry: again, how to measure and what to measure? Comparative Cytogenetics 7(1): 1-9.
Ricroch A, Yockteng R, Brown SC and Nadot S, 2005. Evolution of genome size across some cultivated Allium species. Genome 48: 511-520.
Seijo JG and Fernández A 2003. Karyotype analysis and chromosome evolution in South American species of Lathyrus (Leguminosae). American Journal of Botany 90(7): 980-987.
Singh DC, Sharma R and Laishram JM, 2016. The karyotypic studies of three local shallot of Manipur, India. International Journal of Humanities and Social Sciences 5(2): 17-22.
Stebbins GL, 1971. Chromosomal Evolution in Higher Plants. Addison-Wesley, USA.
Von Bothmer R, 1975. The Allium ampeloprasum complex on Crete. Mitteilungen der Botanischen Staatssammlung München 12: 267-288.
 
Journal of Plant Physiology and Breeding
Volume 9, Issue 2
December 2019
Pages 115-127

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