A key response of grain yield and superoxide dismutase in maize (Zea mays L.) to water deficit stress

Document Type : Research Paper


1 Crop and Horticultural Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Moghan, Iran.

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.


To assess the grain yield, plant height and superoxide dismutase (Cu/Zn-SOD) activity, and its expression responses of maize to water deficit stress, a field experiment using a split-plot design based on randomized complete block design was performed with three maize hybrids, SC704, SC720 and NS640, under control and water-deficit stress conditions at the Research Station of University of Tabriz, Iran. The results indicated that water deficit stress reduced grain yield and plant height. Electrophoretic analysis for Cu/Zn-SOD based on sensitivity to KCN and H2O2 inhibitors was carried out using 8% slab polyacrylamide gels. The gene expression of Cu/Zn-SOD by using Real-time PCR in maize hybrids showed that water deficit stress increased Cu/Zn-SOD expression. SC704 with higher grain yield, plant height and Cu/Zn-SOD activity ranked as a drought-tolerant hybrid in this study. It can be concluded that the increase in Cu/Zn-SOD expression may decrease damage caused by water-deficit stress in maize.


Article Title [Persian]

پاسخ کلیدی عملکرد دانه و سوپراکسید دیسموتاز در ذرت (.Zea mays L) به تنش کم آبی

Abstract [Persian]

به منظور اندازه‌گیری عملکرد دانه، ارتفاع بوته، فعالیت و بیان ژن آنزیم سوپراکسید دیسموتاز (Cu/Zn-SOD)  تحت تنش کم ­آبی، آزمایشی به صورت کرت‌های خرد شده بر پایه بلوک‌های کامل تصادفی با سه هیبرید ذرت (سینگل کراس 704، سینگل کراس، سینگل کراس 640) تحت شرایط مزرعه در ایستگاه تحقیقاتی دانشگاه تبریز اجرا شد. نتایج نشان داد که تنش کم­ آبی باعث کاهش عملکرد دانه و ارتفاع بوته هیبریدهای ذرت شد. آنالیز الکتروفورزی برای Cu/Zn-SOD  براساس حساسیت به مهارکننده پتاسیم سیانید و هیدروژن پراکسید روی ژل پلی آکریلامید هشت درصد انجام گرفت. فعالیت Cu/Zn-SOD تحت تنش کم ­آبی در هیبریدهای ذرت افزایش یافت. بیان نسبی ژن Cu/Zn-SOD به روش  Real-time PCRدر هیبریدهای ذرت نشان داد که تنش کم ­آبی باعث افزایش بیان نسبی Cu/Zn-SOD شد. بر این اساس، سینگل کراس 704 با بیشترین عملکرد دانه، ارتفاع بوته و فعالیت Cu/Zn-SOD به عنوان هیبرید متحمل به خشکی شناسایی شد. بر مبنای نتایج حاصل چنین می‌توان نتیجه گرفت که افزایش بیان نسبی Cu/Zn-SOD می‌تواند صدمات ناشی از تنش کم­ آبی را در ذرت کاهش دهد.

Keywords [Persian]

  • واژه های کلیدی: ارتفاع بوته
  • الکتروفورز
  • آنزیم آنتی اکسیدان
  • ایزوفرم
  • ژن
  • عملکرد دانه
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