Mapping QTLs with additive effects and additive × additive epistatic interactions for harvest index in wheat (Triticum aestivum L.)

Document Type : Research Paper

Authors

1 Sugar Beet Research Department, Agricultural and Natural Resources Research Center of Hamedan, Agricultural Research, Education and Extension Organization (AREEO), Hamedan, Iran.

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

3 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

4 Seed and Plant Improvement Research Department, West Azerbaijan Agricultural and Natural Resources Research Center, AREEO, Urmia, Iran.

5 Department of Agronomy and Plant Breeding, Faculty of Agriculture University of Maragheh, Maragheh Iran.

Abstract

To map quantitative trait loci (QTL) for harvest index, 148 recombinant inbred lines and their parents, Yecora Rojo and an Iranian landrace line (No. 49), were evaluated under normal and terminal water deficit stress conditions in the research stations of Mahabad University and Miyandoab Agricultural Research Center, Iran, during 2014 and 2015. The experiment was carried out as design alpha lattice design with two replications. A linkage map of 51 retrotransposon and 177 microsatellite markers was used in this investigation. Quantitative trait loci (QTL) for additive effects and additive × additive interactions were determined by QTL Network 2.0 software using CIM and mixed-linear methods. QTL analysis revealed that under normal condition, six QTLs (R2A= 0.04 to 12.0%), two QTL × environment (E) interactions (R2AE= 6.28%), five additive × additive epistatic effects (R2AA= 0.7 to 8.68%) and 12 additive × additive × E (R2AAE= 3.76 to 11.4%) were significant. Under water deficit stress conditions, two QTLs (R2A= 5.0 to 7.0%), two additive × additive interactions (R2AA= 3.72 to 5.48%) and seven additive × additive × E interactions (R2AAE= 8.04 to 9.58) were identified. Among the eight QTLs identified, three QTLs were located on chromosome 2D, suggesting the importance of this chromosome in controlling harvest index, which may be used for marker assisted selection in breeding programs.

Keywords

Article Title [فارسی]

مکان‌یابی QTLهای با اثرهای افزایشی و اثرهای متقابل اپیستازی افزایشی × افزایشی برای شاخص برداشت در گندم (.Triticum aestivum L)

Abstract [فارسی]

به ‌منظور مکان‌یابی ژن­های کنترل کننده صفات کمی (QTL) برای شاخص برداشت، 148 لاین اینبرد نوترکیب گندم همراه با والدین ( YecoraRojo و یک لاین محلی ایرانی  با نام No. 49) در شرایط نرمال و تنش کم‌آبی انتهای فصل مورد ارزیابی قرار گرفتند. طرح آزمایشی به صورت آلفا لاتیس با دو تکرار بود که در ایستگاه­ های تحقیقاتی دانشگاه مهاباد و مرکز تحقیقات کشاورزی میاندوآب در طی دو سال زراعی 1393 و  1394 پیاده شد. نقشه پیوستگی مورد استفاده شامل 177 نشانگر ریز ماهواره و 51 نشانگر رتروترانسپوزون بود. مکان­ یابی ژن­ های کنترل کننده صفات کمی برای اثرهای افزایشی و اثرهای متقابل اپیستازی افزایشی × افزایشی با استفاده از نرم افزار QTL Network 2.0 و بر اساس روش مکان­ یابی فاصله ­ای مرکب  و مدل­ های خطی مخلوط صورت گرفت. نتایج تجزیه QTL نشان داد که در شرایط نرمال شش QTL (R2A = 04% تا 12%)، دو اثر متقابل QTL × محیط (R2AE= 6/28%)، پنچ اثر متقابل اپیستازی افزایشی × افزایشی (R2AA = 0/7% تا 8/68%) و 12 اثر متقابل افزایشی × افزایشی × محیط  (R2AAE= 3/76% تا 11/41%) معنی­ دار بودند. تحت شرایط تنش کم آبی دو QTL (R2A = 5% تا 7%)، دو  اثر متقابل اپیستازی افزایشی × افزایشی (R2AA= 3/72% تا 5/48%) و هفت اثر متقابل افزایشی × افزایشی × محیط  (R2AAE= 8/04% تا 9/58%) شناسایی شدند. در مطالعه حاضر سه QTL از هشت QTL شناسایی شده روی کروموزوم شماره 2D مکان­ یابی شد که بیانگر اهمیت این کروموزوم در کنترل شاخص برداشت و امکان استفاده احتمالی از آن در گزینش به کمک نشاگر بود.

Keywords [فارسی]

  • اپیستازی
  • رتروترانسپوزون
  • شاخص برداشت
  • گندم
  • نشانگر ریزماهواره
  • QTL

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Journal of Plant Physiology and Breeding
Volume 9, Issue 2
December 2019
Pages 67-76

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