Influence of textile dyes on some morphological, biochemical and physiological characteristics of broad bean (Vicia faba L.)

Document Type : Research Paper


1 Department of Plant Ecophysiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 Faculty of Chemical and Petroleum Engineering, University of Tabriz, Tabriz, Iran.

3 Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.


An experiment was conducted to evaluate the effect of textile dyes on some morphological and biochemical traits of board bean at a greenhouse of University of Tabriz, Iran, in 2016. Three types of dye (Acid Yellow, Acid Red, Direct Blue) and five dye concentrations (0, 30, 50, 70 and 90 mg/L) were studied using a factorial experiment based on randomized complete block design with three replications. Treatments were applied at seedling, pre-flowering and flowering stages. In this experiment, seed number, leaf number, peroxidase (POX) and catalase (CAT) activities, decolorization percentage and protein content were measured at all three stages of development.  Results indicated that the effect of dye concentrations was significant on POX, CAT, leaf number, seed number and decolorization percentage at pre-flowering and flowering stages, and on protein content at all stages, whereas type of dye only had significant effect on seed number, protein content at pre-flowering and on CAT activity at both pre-flowering and flowering stages. Interaction of dye type with dye concentration was only significant for decolorization percentage at pre-flowering and flowering stages. The greatest increase in POX and CAT activities, and protein content were observed with 90 mg/L dye concentration at both pre-flowering and flowering stages; however, highest seed number were observed with the control treatment (0 mg/L dye concentrations) at pre-flowering and flowering stages. We may conclude that although absorbing dye by plants imposed a stress on them, but they were able to survive under this stress. Therefore, we might consider broad bean as one of the efficient plants for phytoremediation.


Article Title [Persian]

تاثیر مواد رنگزا بر برخی صفات مورفولوژیک، بیوشیمیایی و فیزیولوژیک باقلا (.Vicia faba L)

Abstract [Persian]

به منظور بررسی گیاه پالایی پساب رنگی توسط باقلا و اثرات رنگ بر این گیاه آزمایشی به صورت فاکتوریل در قالب طرح بلوک­­ های کامل تصادفی با سه تکرار در سال­ زراعی 1395 در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه تبریز، به اجرا در ­آمد. فاکتور اول نوع رنگ در سه سطح، شامل رنگ زرد اسیدی (Acid Yellow)، قرمز اسیدی (Acid Red) و آبی دایرکت (Direct Blue) و فاکتور دوم غلظت رنگ در پنج سطح شامل صفر، 30، 50، 70 و 90 میلی گرم بر لیتر ماده رنگی بود. تیمارها در سه مرحله گیاهچه ­­ای، قبل از گلدهی و گلدهی ارزیابی شدند. در این پژوهش برخی صفات شامل درصد تجزیه رنگ، تعداد دانه، تعداد برگ، میزان پروتئین و فعالیت پراکسیداز و کاتالاز مورد اندازه­­ گیری قرار گرفتند. اثر غلظت رنگ در مراحل قبل از گلدهی و گلدهی بر میزان پراکسیداز، کاتالاز، تعداد برگ، تعداد دانه و درصد تجزیه رنگ و در هر سه مرحله روی میزان پروتئین معنی­­ دار بود، در حالی که  نوع رنگ فقط بر تعداد دانه و میزان پروتئین در مرحله قبل از گلدهی و فعالیت کاتالاز در هر دو مرحله قبل از گلدهی و گلدهی تأثیر معنی ­داری داشت. اثر متقابل نوع رنگ با غلظت رنگ فقط در مورد درصد تجزیه رنگ، در مراحل قبل از گلدهی و گلدهی، معنی­­ دار  شد. بیشترین میزان فعالیت­  POX و CAT و درصد پروتئین با غلظت رنگ 90 میلی گرم در لیتر در هر دو مرحله قبل از گلدهی و گلدهی مشاهده شد؛ با این حال، بیشترین تعداد دانه در غلظت صفر میلی گرم در لیتر رنگ در مراحل قبل از گلدهی و گلدهی به دست آمد. می­­ توان نتیجه گرفت که اگرچه جذب رنگ توسط باقلا مقداری تنش بر آن تحمیل کرد، ولی این گیاه توانست تحت این تنش زنده بماند. بنابراین، ممکن است گیاه باقلا را به عنوان یکی از گیاهان کارآمد برای گیاه ­­پالایی توصیه کرد.

Keywords [Persian]

  • باقلا
  • تعداد دانه
  • رنگزا
  • رنگ زدایی
  • گیاه پالایی
Aebi H, 1984. Catalase in vitro. Methods in Enzymology 105: 121-126.
Anjana S and Salom Gnana Thanga V, 2011. Phytoremediation of synthetic textile dyes. Asian Journal of Microbiology, Biotechnology and Environmental Sciences 13(1): 30-39.
Asamudo NU, Daba AS and Ezeronye OU, 2005. Bioremediation of textile effluent using Phanerochaete chrysosporium. African Journal of Biotechnology 4(13): 1548-1553.
Bradford M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 7(72): 248-254.
Dutta SK and Boissya CL, 2000. Effect of Nagaon paper mill (Jagiroad, Assam) effluent on the yield components of rice (Oryza sativa L. var. Mahsuri). Ecology, Environment and Conservation 6(4): 453-457.
Gabara B, Sklodowska M, Wyrwicka A, Glinska S and Gapińska M, 2003. Changes in the ultrastructure of chloroplasts and mitochondria and antioxidant enzyme activity in Lycopersicon esculentum Mill. leaves sprayed with acid rain. Plant Science 164(4): 507-516.
Herzog V and Fahimi H, 1973. Determination of the activity of peroxidase. Analytical Biochemistry Journal 55: 554-562.
Jayanthy V, Geetha R, Rajendran R, Prabhavathi P, Karthik Sundaram S, Dinesh Kumar S and Santhanam P, 2013. Phytoremediation of dye contaminated soil by Leucaena leucocephala (subabul), and seed and growth assessment of Vigna radiata in the remediated soil. Saudi Journal of Biological Sciences 21(4): 324-334.
Kabra AN, Khandare RV, Waghmode TR and Govindwar SP, 2012. Phytoremediation of textile effluent and mixture of structurally different dyes by Glandularia pulchella (Sweet) Tronc. Chemosphere 87(3): 265-272.
Khandare RV, Kabra AN, Tamboli DP and Govindwar SP, 2011. The role of Aster amellus Linn. in the degradation of a sulfonated azo dye Remazol Red: a phytoremediation strategy. Chemosphere 82(8): 1147-1154.
Khataee AR, Movafeghi A, Torbati S, Salehi Lisar SY and Zarei M, 2012.  Phytoremediation potential of duckweed (Lemna minor L.) in degradation of C.I. Acid Blue 92: artificial neural network modeling. Ecotoxicology and Environmental Safety 80: 291-298.
Khataee AR, Movafeghi A, Vafaei F, Lisar SYS and Zarei M, 2013. Potential of the aquatic fern Azolla filiculoides in biodegradation of an azo dye: modeling of experimental results by artificial neural networks. International Journal of Phytoremediation 15(8): 729-742.
Lokhande VH, Kudale S, Nikalje G, Desai N and Suprasanna P, 2015. Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L. Biotechnology Reports 8: 56-63.
Mahmood Q, Masood F, Bhatti ZA, Siddique M, Bilal M and Yaqoob H, 2014. Biological treatment of the dye Reactive Blue 19 by cattails and anaerobic bacterial consortia. Toxicological and Environmental Chemistry 96(4): 530-541.
Mittler R, 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7(9): 405-410.
Noctor G, Veljovic-Jovanovic S and Foyer CH, 2000. Peroxide processing in photosynthesis: antioxidant coupling and redox signalling. Philosophical Transactions of The Royal Society of London B-Biological Sciences 355(1402): 1465-1475.
Olejnik D and Wojciechowski K, 2012. The conception of constructed wetland for dyes removal in water solutions. CHEMIK 66(6): 611-614
Patil AV and Jadhav JP, 2012. Evaluation of phytoremediation potential of Tagetes patula L. for the degradation of textile dye Reactive Blue 160 and assessment of the toxicity of degraded metabolites by cytogenotoxicity. Chemosphere 92(2): 225-232.
Ramya S, Pradeep Kumar R, Murugesan S and Anitha S, 2017. Effect of textile effluent on seedling germination, growth and biochemical characteristics of Arachis hypogaea. l.  Variety K6.  International Journal of Pharma Research and Health Sciences 5(4): 1805-1809.
Ravi D, Parthasarathy R, Vijayabharathi V and Suresh S, 2014. Effect of textile dye effluent on soybean crop. Journal of Pharmaceutical, Chemical and Biological Sciences 2(2): 111-117.
Salakinkop SR and Hunshal, 2014. CS Domestic sewage irrigation on dynamics of nutrients and heavy metals in soil and wheat (Triticum aestivum L.) production. International Journal of Recycling of Organic Waste in Agriculture 3(8).
Scandalios JG, Guan LM and Polidoros A, 1997. Catalase in plants: gene structure, properties, regulation, and expression. In: Scandalios JG (ed.) Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Pp. 343-406. Cold Spring Harbor Laboratory Press, Plainview, New York, USA.
Sivakumar K, Subbaiah KV and Sai Gopal DVR, 2001. Studies of certain trace elements in industrial effluents, sediments and their effect on plant physiology. Pollution Research 20(1): 99-102.
Sudhakar C, Lakshmi A and Giridarakumar S, 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science 161 (3): 613-619.
Swaminathan L, Manonmani K and Sarojini B, 1992. Studies on the toxicity of South Indian Viscose factory effluent on groundnut Arachis hypogea. Journal of Environmental Biology 13(3): 253-260.
Torbati S, Khataee AR and Movafeghi A, 2014. Application of watercress (Nasturtium officinale R. Br.) for biotreatment of a textile dye: investigation of some physiological responses and effects of operational parameters. Chemical Engineering Research and Design 92(10): 1934-1941.
Vafaei F, Movafeghi A and Khataee A, 2013. Evaluation of antioxidant enzymes activities and identification of intermediate products during phytoremediation of an anionic dye (C.I. Acid Blue 92) by pennywort (Hydrocotyle vulgaris). Journal of Environmental Sciences 25(11): 2214-2222.
Watharkar AD and Jadhav JP, 2014. Detoxification and decolorization of a simulated textile dye mixture by phytoremediation using Petunia grandiflora and, Gailardia grandiflora: a plant–plant consortial strategy. Ecotoxicology and Environmental Safety 103: 1-8.
Yasmeen T, Ali Q, Islam F, Noman A, Sohail Akram M and Tariq Javed M, 2014. Biologically treated wastewater fertigation induced growth and yield enhancement effects in Vigna radiata L. Agricultural Water Management 146: 124-130.