Effects of seed priming with salicylic acid on chlorophyll a fluorescence parameters of basil (Ocimum basilicum L.) infested by field dodder (Cuscuta campestris Yunk.)

Document Type : Research Paper


1 PhD student of Crop Physiology, Department of Plant Eco-Physiology, University of Tabriz, Tabriz, Iran.

2 Department of Plant Eco-Physiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

3 Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.


This research was undertaken to assess the ameliorative effect of salicylic acid (0.5 mM) on photosystem II (PS II) in two basil (Ocimum basilicum L.) varieties (Iranian and Italian) infested with field dodder (Cuscuta campestris Yunk.) in the greenhouse of University of Tabriz, Iran, in 2017. The treatments were arranged as factorial using randomized complete block design with four replications. Results indicated that application of salicylic acid improved the maximum quantum efficiency of photosystem II (Fv/Fm) and performance index (PI) of basil plants infested by field dodder. The time span from F0 to Fm (TFM) and the energy necessary for the closure of all reaction centers (Sm) were significantly increased and the size of the plastoquinone pool (Area) declined in plants exposed to field dodder infestation. Application of salicylic acid increased the chlorophyll a fluorescence parameters studied in both basil varieties, especially in the Italian variety.


Article Title [فارسی]

اثر پرایمینگ بذر با اسید سالیسیلیک روی پارامترهای فلورسانس کلروفیل a در ریحان آلوده به سس (Cuscuta campestris Yunk.)

Abstract [فارسی]

این تحقیق به منظور ارزیابی اثر بهبود دهنده اسید سالیسیلیک (5/0 میلی مولار) بر فتوسیستم II (PS II) در دو رقم ریحان (Ocimum basilicum L.) (ایرانی و ایتالیایی) آلوده به علف­ هرز سس مزرعه (Cuscuta campestris Yunk.) در شرایط گلخانه در دانشگاه تبریز در سال 1396 انجام شد. آزمایش در قالب فاکتوریل بر پایه طرح بلوک‌های کامل تصادفی در چهار تکرار  پیاده شد. نتایج نشان داد که کار برد اسید سالیسیلیک، حداکثر کارایی کوانتومی فتوسیستم II (Fv/Fm) و شاخص عملکرد فتوسنتزی (PI) را در ریحان آلوده به سس بهبود داد. در گیاهان آلوده به سس، مدت زمان لازم بین فلورسانس حداقل و حداکثر (TFM) و انرژی لازم برای بسته شدن مراکز واکنش (Sm) به طور معنی­ داری افزایش و میزان پلاستوکوئینون موجود (Area) کاهش یافت. استفاده از اسید سالیسیلیک پارامترهای مورد مطالعه فلورسانس کلروفیل a را در هر دو رقم ریحان افزایش داد، ولی این افزایش در رقم ایتالیایی بیشتر بود.

Keywords [فارسی]

  • اسید سالیسیلیک
  • پرایمینگ بذر
  • ریحان
  • سس
  • فلورسانس کلروفیل a
Aly R, 2013. Trafficking of molecules between parasitic plants and their hosts. Weed Research 53(4): 231-241.
Bayat H, Alirezaie M and Neamati H, 2012. Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress. Journal of Stress Physiology and Biochemistry 8(1): 258-267.
Behbahani M, 2014. Evaluation of in vitro anticancer activity of Ocimum basilicum, Alhagi maurorum, Calendula officinalis and their parasite Cuscuta campestris. PLoS ONE 9: 10.1371/journal.pone.0116049.
Browicz K, 1982. Chorology of Trees and Shrubs in South-West Asia and Adjacent Regions. Vol. 1. Polish Scientific Publishers, Poznan, Poland.
Cameron DD, Geniez J-M, Seel WE  and Irving LJ, 2008. Suppression of host photosynthesis by the parasitic plant Rhinanthus minor. Annals of Botany 101(4): 573-578.
Farooq M, Aziz T, Basra SMA, Cheema MA and Rehman H, 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. Journal of Agronomy and Crop Science 194(2): 161-168.
Gharib FA and Hegazi AZ, 2010. Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolus vulgaris L.) under cold stress. Journal of American Science 6(10): 675-683.
Ghassemi-Golezani K and Lotfi R, 2015. The impact of salicylic acid and silicon on chlorophyll a fluorescence in mung bean under salt stress. Russian Journal of Plant Physiology 62: 611-616.
Gornik K and Lahuta LB, 2017. Application of phytohormones during seed hydropriming and heat shock treatment on sunflower (Helianthus annuus L.) chilling resistance and changes in soluble carbohydrates. Acta Physiologiae Plantarum 39118. https://doi.org/10.1007/s11738-017-2413-x.
Hayat Q, Hayat S, Alyemeni MN and Ahmad A, 2012. Salicylic acid mediated changes in growth, photosynthesis, nitrogen metabolism and antioxidant defense system in Cicer arietinum L. Plant, Soil and Environment 58(9): 417-423.
He Y and Zhu ZJ, 2008. Exogenous salicylic acid alleviates NaCl toxicity and increases antioxidative enzyme activity in Lycopersicon esculentum. Biologia Plantarum 52: 792-795.
Janda T, Gondor OK, Yordanova R, Szalai G and Pal M, 2014. Salicylic acid and photosynthesis: signalling and effects. Acta Physiologiae Plantarum 36(10): 2537-2546.
Janda T, Szalai G, Antunovics Zs, Ducruet J-M and Paldi E, 1998. Effects of salicylic acid and related compounds on photosynthetic parameters in young maize (Zea mays L.) plants. In: Garab G (eds.) Photosynthesis: Mechanisms and Effects. Pp. 3869-3872. Kluwer Academic Publishers, Dordrecht, Netherlands.
Janda T, Szalai G, Antunovics Zs, Horvath E and Paldi E, 2000. Effect of benzoic acid and aspirin on chilling tolerance and photosynthesis in young maize plants. Maydica 45(1): 29-33.
Kalaji HM, Govindjee B, Bosa K, Koscielniak J and Zuk-Gołaszewska K, 2011. Effects of salt stress on photosystem II efficiency and CO2 assimilation of two Syrian barley landraces. Environmental and Experimental Botany 73: 64-72.
Krause GH and Weis E, 1984. Chlorophyll fluorescence as a tool in plant physiology: II. Interpretation of fluorescence signals. Photosynthesis Research 5: 139-157.
Lotfi R, Pessarakli M Gharavi-Kouchebagh P and Khoshvaghti H, 2015. Physiological responses of Brassica napus to fulvic acid under water stress: chlorophyll a fluorescence and antioxidant enzyme activity. The Crop Journal 3(5): 434-439.
Luna E, Bruce TJA, Roberts MR, Flors V and Ton J, 2012. Next-generation systemic acquired resistance. Plant Physiology 158(2): 844-853.
Mehta P, Jajoo A, Mathur S, and Bharti S, 2010. Chlorophyll a fluorescence study revealing effects of high salt stress on photosystem II in wheat leaves. Plant Physiology and Biochemistry 48(1): 16-20.  
Munné-Bosch S and Peñuelas J, 2003. Photo- and antioxidative protection, and a role for salicylic acid during drought and recovery in field-grown Phillyrea angustifolia plants. Planta 217: 758-766.
Nawrath C, Métraux JP and Genoud T, 2005. Chemical signals in plant resistance: salicylic acid. In Tuzun S and Bent E (Eds.) Multigenic and Induced Systemic Resistance in Plants. Pp. 143-165. Springer, Dordrecht, Netherlands.
Nimir NEA, Lu S, Zhou G, Guo W, Ma B and Wang Y, 2015. Comparative effects of gibberellic acid, kinetin and salicylic acid on emergence, seedling growth and the antioxidant defence system of sweet sorghum (Sorghum bicolor) under salinity and temperature stresses. Crop and Pasture Science 66(2): 145-157.
Pouramir-Dashtmian F, Khajeh-Hosseini M and Esfahani M, 2014. Improving chilling tolerance of rice seedling by seed priming with salicylic acid. Archives of Agronomy and Soil Science 60(9): 1291-1302.
Saric-Krsmanovic M, Bozic D, Radivojevic Lj, Gajic Umiljendic JG and Vrbnicanin S, 2018. Impact of field dodder (Cuscuta campestris Yunk.) on chlorophyll fluorescence and chlorophyll content of alfalfa and sugar beet plants. Russian Journal of Plant Physiology 65: 726-731.
Szepesi A, Csiszar J, Bajkan Sz, Gemes K, Horvath F, Erdei L, Deer A, Simon LM and Tari I, 2005. Role of salicylic acid pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis 49: 123-125.
Tewari D, Sah AN, Pandey HK and Meena HS, 2012: A review on phytoconstituents of Ocimum (Tulsi). International Journal of Ayurvedic Medicine 3(1): 1-9.