Salinity stress responsive leaf proteins in alfalfa (Medicago sativa L.)

Document Type : Research Paper


1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 Department of Plant Ecophysiology, Faculty of Agriculture University of Tabriz, Tabriz, Iran.


Salinity stress is one of the most harmful abiotic stresses that affect agronomical, physiological and biochemical processes in crop plants. In order to evaluate the effect of salinity stress on alfalfa (Bami ecotype) leaf proteins based on two-dimensional gel electrophoresis, an experiment was conducted with two salinity treatments (0 and 200 mM NaCl) and six replications under hydroponic cultural system. Salinity stress decreased fresh and dry weight of alfalfa about 24.5 and 39.5 percent, respectively. Proteome analysis was carried out on the leaf tissue using three replications. Fourteen repeatable protein spots had significant change in expression under salt stress. Probabilistic identification of proteins from the data bank was performed by isoelectric point and molecular weight. Nine proteins showed significant up-regulation and five proteins had down regulation. Candidate proteins were among the proteins involved in the defense system, regulation, metabolic pathways, nitrogen fixation and canalization. Higher abundance of trehalose-phosphate phosphatase 2, involved in regulation, and also uracil phosphoribosyl transferase lastic and β-hydroxyisobutyryl-CoA hydrolase 1, involved in the energy metabolism, demonstrated the important function of these proteins under salinity stress. It seems that the alfalfa plant manages to reduce the adverse effects of salinity through the candidate proteins identified in this investigation.


Article Title [فارسی]

پروتئین های پاسخ دهنده به تنش شوری در یونجه (.Medicago sativa L)

Abstract [فارسی]

تنش شوری یکی از مهم­ترین تنش­ های محیطی است که روی صفات زراعی، فیزیولوژیکی و بیوشیمیایی گیاه تاثیر می­ گذارد. به منظور ارزیابی اثر تنش شوری (صفر و 200 میلی ­مولار کلرید سدیم) روی پروتئین­ های برگ یونجه به روش الکتروفورز دوبُعدی، آزمایشی با دو تیمار شوری (سطح صفر و 200 میلی­ مولار کلرید سدیم) با شش تکرار تحت شرایط هیدروپونیک اجرا شد. تنش شوری باعث کاهش 5/24 و 5/39 درصد وزن تر و وزن خشک گیاهچه­ ها در  اکوتیپ بمی یونجه شد. شناسایی احتمالی پروتئین­ ها توسط  pIو وزن ملکولی تقریبی انجام شد. نتایج حاصل از تجزیه پروتئوم برگ یونجه نشان داد که 14 لکه تکرار پذیر تحت تنش شوری تغییر بیان داشتند به طوری که 9 لکه پروتئینی افزایش بیان و پنج لکه پروتئینی کاهش بیان نشان دادند. پروتئین­ های کاندید جزء پروتئین­ های دخیل در سیستم دفاعی، تنظیم، مسیرهای متابولیسم، تثبیت نیتروژن و کانالی بودند. بیان بالاتر trehalose-phosphate phosphatase 2، پروتئین درگیر در عمل تنظیم، و نیز uracil phosphoribosyl transferase, lastic  و β-hydroxyisobutyryl-CoA hydrolase 1، پروتئین­ های درگیر در متابولیسم انرژی، عملکرد مهم این پروتئین ­ها را تحت تنش شوری نشان داد. چنین به نظر می­ رسد که گیاه یونجه از طریق پروتئین­ های کاندید مشخص شده در این پژوهش موجب کاهش اثر تنش شوری می­ شود.

Keywords [فارسی]

  • پروتئوم
  • تنش غیرزیستی
  • ژل دوبُعدی
  • سیستم دفاعی
  • متابولیسم
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