Contamination control in Iranian seedless barberry micropropagation

Document Type : Research Paper

Authors

1 Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Khorasan Science and Technology Park (KSTP), Mashhad, Iran.

3 Department of Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Iranian seedless barberry is a species with high degree of contamination in in vitro culture. The current study was aimed to investigate the effects of mercuric chloride (HgCl2), sodium hypochlorite (NaOCl) + HgCl2, HgCl2 + 8-hydroxyquinoline, cefotaxime + streptomycin, HgCl2 + shoot type, HgCl2 + explant position and HgCl2 + shoot color on the reduction of contamination. The results showed that the combined use of NaOCl and HgCl2 was more effective than their single use, but the contamination rate was relatively high. Interestingly, the results showed that explants sterilized with 100 mg/L HgCl2 and cultured in the medium containing 150 mg/L 8-hydroxyquinoline exhibited the lowest fungal contamination (15%), while those treated with 50 mg/L HgCl2 and cultured in the 8-hydroxyquinoline-free medium showed the highest fungal contamination (96%). Furthermore, treatment of 75 mg/Lcefotaxime + 70 mg/L streptomycin exhibited no bacterial contamination compared to the control with 58% bacterial contamination. Explants prepared from suckers and treated with 150 mg/L HgCl2 showed the lowest rates of fungal (16%) and bacterial (13%) contamination. The results also showed that explants collected from upper position had the lowest rates of fungal (20.87%) and bacterial (18.4%) infection. Explants taken from pinkish-colored shoots showed surprisingly the lowest rates of fungal contamination (13.33%), bacterial contamination (10%) and bud mortality (36.47%). In general, choice of explants from upper position of pinkish-colored suckers, sterilization of them with 100 mg/L HgCl2 for 5 min and their culture in medium containing 150 mg/L 8-hydroxyquinoline can be recommended for reducing the contamination.

Keywords

References

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Journal of Plant Physiology and Breeding
Volume 9, Issue 1
June 2019
Pages 97-110

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