Proteomics analysis of canola seeds to identify differentially expressed proteins under salt stress

Document Type : Research Paper

Authors

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Abstract

Seeds are an important part of the canola plant, and similar to other parts are affected by salt stress. Understanding the underlying mechanisms that take place in seeds of canola under salt stress is essential from the perspective of improving quality. In this study, we attempted to identify differentially expressed proteins of canola seeds in the Hyola308 cultivar under 350 mM NaCl using two dimensional (2D) gel electrophoresis. Effect of salt stress was significant on 100-seed weight and number of seeds per plant (p≤ 0.01), and it changed the proteome of the seeds. From a total of 548 reproducible protein spots, 28 protein spots showed significant changes in abundance, of which seven spots showed downregulation. The “Gene Ontology” analysis classified differentially expressed proteins into six biological processes: oxidation-reduction (28.5%), response to abiotic stress (28.5%), response to hormones (21.4%), catabolic process (21.4%), nucleoside diphosphate phosphorylation (17.8%) and glycolytic process (14.28%). In conclusion, salt stress induced canola seeds to upregulate proteins that mostly involved in the antioxidant activity and the proteins with nutrient reservoir activity.

Keywords


Article Title [فارسی]

تجزیه پروتئوم بذور کلزا برای شناسائی پروتئین‌های تغییر بیان یافته تحت تنش شوری

Abstract [فارسی]

بذور همانند سایر قسمت‌های گیاه بخش مهمی هستند که توسط تنش شوری تحت تاثیر قرار می‌گیرند. درک سازوکار‌های مولکولی که در بذور گیاه تحت تنش شوری اتفاق می‌افتد، از لحاظ توسعه کیفی اهمیت دارد. در این مطالعه، با استفاده از االکتروفورز ژل دو بعدی (2-DE)تلاش شده است تا پروتئین‌های با تغییرات بیان معنی­دار بذور کلزا (رقم Hyola308) تحت تنش شوری شناسایی شود. نتایج نشان داد که تاثیر تنش شوری روی وزن صد دانه و تعداد دانه در سطح احتمال  یک درصد معنی­دار است و پروتئوم بذور متاثر از تنش می‌باشد. در کل از 548 لکه پروتئینی تکرارپذیر، 28 لکه پروتئینی تغییر بیان معنی­دار نشان دادند که از آن­ها هفت لکه دارای کاهش بیان بودند. آنالیز   GO(هستی شناسی ژنی) پروتئین‌های با تغییرات بیان معنی‌دار را در شش فرآیند بیولوژیکی تقسیم بندی کرد: فرآیند اکسیداسیون-احیاء (5/28%)، واکنش به تنش غیر­زیستی (5/28%)، واکنش به هورمون (4/21%)، فرآیند کاتابولیکی (4/21%)، فسفوریلاسیون دی فسفاتی نوکلئوزید (8/17%) و فرآیند گلیکولیزی (28/%14). در نهایت تنش شوری موجب تحریک بذور کلزا برای افزایش بیان پروتئین‌های دخیل در فعالیت آنتی اکسیدانی و پروتئین‌های با فعالیت ذخیره‌ای شد.
 

Keywords [فارسی]

  • الکتروفورز ژل پلی‌آکریلامید دو بعدی
  • پروتئومیک
  • تنش غیر‌زیستی
  • فعالیت آنتی اکسیدانی
  • Brassica napus
 
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