Responses of germination characteristics and antioxidant enzymes activity to different levels of hydro-priming and seed ageing in three maize (Zea mays L.) hybrids

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran.

2 Seed and Plant Certification and Registration Research Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

Abstract

Seed storage at unfavorable conditions of high humidity and temperature is a difficulty which decreases seed vigor and/or seed viability. Current experiment was conducted to evaluate the activity of antioxidant enzymes and seed germination characteristics after ageing on maize seeds (Zea mays L.) in the research laboratory of Razi University, Kermanshah, Iran. A three-factorial experiment was performed with completely randomized design with three replications, using three maize hybrids (SC704, SC647, SC260), and three levels of accelerated ageing (non-aged, artificially aged for 3 and 6 days at 45 oC temperature and 98% relative humidity). The hydro-priming was performed at different durations (0, 12 and 24 hours). Results indicated that germination characteristics of maize seeds were considerably affected by priming time periods. The germination characteristics of deteriorated seeds were significantly enhanced by hydro-priming, especially in 24 h priming. The highest germination characteristics and seedling length were obtained in the control (non-aged) seeds which were primed for 24 h. The lowest germination performance was obtained for the non-primed seeds after 6 days of ageing. The catalase, peroxidase and glutathione reductase activity and malondialdehyde (MDA) concentration of aged maize seeds were restored partially by hydro-priming. In fact, ageing-associated MDA decreased by priming. The results suggested that hydro-priming could improve deteriorated seed performance.
 

Keywords

Article Title [Persian]

پاسخ خصوصیات جوانه زنی و فعالیت آنزیم‌های آنتی اکسیدان به سطوح مختلف هیدروپرایم و پیری بذر در سه هیبرید ذرت (Zea mays L.)

Abstract [Persian]

نگهداری بذر در شرایط نامطلوب رطوبت و دمای بالا بسیار دشوار است که باعث کاهش بنیه بذر و یا زنده مانی بذر می­شود. آزمایش حاضر به منظور بررسی فعالیت آنزیم­های آنتی اکسیدان و خصوصیات جوانه زنی پس از پیری روی بذور ذرت (Zea mays L.) در آزمایشگاه تحقیقاتی دانشگاه رازی، کرمانشاه، انجام شد. آزمایش به صورت سه فاکتوره در قالب طرح کاملاً تصادفی با سه تکرار انجام شد که در آن سه هیبرید ذرت (SC704، SC647، SC260) و سه سطح زوال تسریع شده بذر (بدون زوال (سالم)، زوال مصنوعی بذر به مدت 3 و 6 روز در دمای 45 درجه سانتیگراد و رطوبت نسبی 98 درصد) استفاده شد. هیدروپرایمینگ با مدت زمان مختلف (صفر، 12 و 24 ساعت) اعمال شد. نتایج نشان داد که خصوصیات جوانه زنی بذر ذرت به طور معنی­داری تحت تأثیر مدت زمان پرایمینگ قرار گرفت. معیارهای جوانه زنی بذور زوال یافته به طور معنی­داری در اثر هیدروپرایمینگ به خصوص به مدت 24 ساعت افزایش یافت. بیشترین میزان خصوصیات جوانه زنی و طول گیاهچه در بذور سالم (بدون زوال) که به مدت 24 ساعت پرایم شده بود به دست آمد. کمترین میزان جوانه زنی پس از 6 روز اعمال پیری درحالت بدون پرایمینگ حاصل شد. فعالیت کاتالاز، پراکسیداز و گلوتاتیون ردوکتاز و غلظت مالون دی آلدئید (MDA) در بذور زوال یافته با هیدروپرایمینگ بهبود یافت. در واقع میزان MDA که وابسته به پیری است با اعمال پرایم کاهش یافت. نتایج نشان می­دهد که هیدروپرایمینگ می­تواند زوال بذر را بهبود بخشد.
 

Keywords [Persian]

  • پیش تیمار
  • خصوصیات رشدی گیاهچه
  • زوال بذر
  • صفات بیوشیمیایی
  • هیبریدهای ذرت

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Journal of Plant Physiology and Breeding
Volume 9, Issue 1
June 2019
Pages 17-32

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