University of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Growth analysis, agronomic and physiological characteristics of three hybrid varieties of maize under deficit irrigation conditionsآنالیز رشد و خصوصیات زراعی و فیزیولوژیک سه هیبرید ذرت تحت شرایط کم آبیاری1161009710.22034/jppb.2019.10097ENAmir AbbasRostami AjirlooUnit of Agroecology, Department of Agronomy, College of Agriculture, University of Zabol, Zabol, Iran.Mohamad RezaAsgharipourUnit of Agroecology, Department of Agronomy, College of Agriculture, University of Zabol, Zabol, Iran.AhmadGanbariUnit of Agroecology, Department of Agronomy, College of Agriculture, University of Zabol, Zabol, Iran.MahdiJoudiMeshkinshahr College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.MahmoudKhoramivafaDepartment of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.Journal Article20181006Determining appropriate deficit irrigation regimes and varieties under these conditions is necessary to optimize the use of available water in arid and semi-arid regions. In this regard, field experiments were carried out for two years (2014-2015) at the Moghan plain, Iran. The experimental design in each year was split plot based on randomized complete blocks with three replications. The main plots consisted of four irrigation levels: normal irrigation, 80% of maximum daily crop evapotranspiration (ETc), 70% ETc and 50% ETc. Three maize hybrids (SC704, SC703, SC705) were arranged in the sub-plots. Mean comparisons showed that deficit irrigation caused a significant decrease in grain yield and other agronomic traits, physiological characteristics (chlorophyll a, b and relative water content) and growth parameters (leaf area index, crop growth rate, relative growth rate). On the other hand, leaf rolling percentage increased due to the water deficit stress. By increasing deficit irrigation intensity (especially at 50% ETc), chlorophyll a, b and relative water content decreased in SC705 more than the other two hybrids (SC703, SC704) and the leaf rolling percentage at the severe stress condition reached to 60% in SC705. The highest grain yield (8.51 t/ha) and biomass (19.36 t/ha), averaged over two years, were observed under normal irrigation for the SC705 hybrid. However, this hybrid had minimum grain yield and biomass at 50% ETc. By increasing water deficit from normal irrigation to 50% ETc, significant decrease was observed for leaf area index, plant growth rate, relative growth rate and net photosynthesis rate of SC705. Due to the sensitivity of physiological characteristics and growth parameters to the deficit irrigation and the influence of grain yield from these traits, it seems necessary to prevent the occurrence of water deficit at critical stages of maize growth. In conclusion, due to the sensitivity of the SC705 to water shortage, this hybrid is not recommended to water deficit conditions and it would rather be planted when enough irrigation water is available. On the other hand, SC704 seems suitable for the water deficit environments, especially at the severe water stress condition (50% ETc).<br /> <br /> https://breeding.tabrizu.ac.ir/article_10097_3a54ac7f8528b7b8e8a730765fc5e1f7.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Responses of germination characteristics and antioxidant enzymes activity to different levels of hydro-priming and seed ageing in three maize (Zea mays L.) hybridsپاسخ خصوصیات جوانه زنی و فعالیت آنزیمهای آنتی اکسیدان به سطوح مختلف هیدروپرایم و پیری بذر در سه هیبرید ذرت (Zea mays L.)17321009910.22034/jppb.2019.10099ENMaryamGoodarzian GhahfarokhiDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran.BabakDarvishiSeed and Plant Certification and Registration Research Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.MajidAbdoliYoung Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran.0000-0001-8877-1915Journal Article20180902Seed storage at unfavorable conditions of high humidity and temperature is a difficulty which decreases seed vigor and/or seed viability. Current experiment was conducted to evaluate the activity of antioxidant enzymes and seed germination characteristics after ageing on maize seeds (<em>Zea mays</em> L.) in the research laboratory of Razi University, Kermanshah, Iran. A three-factorial experiment was performed with completely randomized design with three replications, using three maize hybrids (SC704, SC647, SC260), and three levels of accelerated ageing (non-aged, artificially aged for 3 and 6 days at 45 <sup>o</sup>C temperature and 98% relative humidity). The hydro-priming was performed at different durations (0, 12 and 24 hours). Results indicated that germination characteristics of maize seeds were considerably affected by priming time periods. The germination characteristics of deteriorated seeds were significantly enhanced by hydro-priming, especially in 24 h priming. The highest germination characteristics and seedling length were obtained in the control (non-aged) seeds which were primed for 24 h. The lowest germination performance was obtained for the non-primed seeds after 6 days of ageing. The catalase, peroxidase and glutathione reductase activity and malondialdehyde (MDA) concentration of aged maize seeds were restored partially by hydro-priming. In fact, ageing-associated MDA decreased by priming. The results suggested that hydro-priming could improve deteriorated seed performance.<br /> https://breeding.tabrizu.ac.ir/article_10099_039ce834dfd5008e4c4fbaacc3c17ff5.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601The effects of soil cover and Stimplex (Ascophyllum nodosum extract) foliar application on antioxidant activity and some physiological characteristics of Coriandrum sativum L.تاثیر خاکپوش و محلولپاشی برگی با استیمپلکس (Ascophyllum nodosum) بر ویژگیهای آنتیاکسیدانی و برخی ویژگیهای فیزیولوژیک گشنیز33451010010.22034/jppb.2019.10100ENLamiaVojodi MehrabaniDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.RanaValizadeh KamranDepartment of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.Journal Article20180903Two field experiments were conducted as factorial based on randomized complete block design to study the effects of soil covering (black, white, control), location (Sarab and Shabestr, Iran), and Stimplex foliar application levels (0, 2, 4 and 6 ml L<sup>-1</sup>) on growth and physiological traits of <em>Coriandrum sativum</em><em>.</em> The highest values for essential oil, flavonoids and nitrogen contents were recorded for black covering at 2 ml L<sup>-1</sup> foliar application of Stimplex. For phenolic compounds the highest values were observed with black covering at 2, 4 and 6 ml L<sup>-1</sup> of foliar spray. In the city of Sarab, maximum values of root dry weight and essential oil content were obtained with 6 and 2 ml L<sup>-1</sup> Stimplex spray, respectively. The best IC<sub>50</sub> belonged to the white and black soil covers in Shabestar (1.6 and 1.7 mg ml<sup>-1</sup>, respectively). Foliar spray had significant effects on the above-ground biomass, plant height, axillary brunch number, umbel number, total soluble solids (TSS), phosphorus and potassium contents. Sarab had higher values than Shabestar for the nitrogen and potassium contents, axillary brunch number and TSS. Black soil cover had positive effects on K (%), P (%), TSS, chlorophyll a, umbel number per plant, plant height, axillary brunch number and above-ground biomass. In total, the results revealed that using black soil cover and foliar application of algal extract (2 ml.L<sup>-1</sup>) improved the growth and physiological responses of <em>C. sativum</em> plants.<br /> https://breeding.tabrizu.ac.ir/article_10100_55c42ecf8d85f9ecf99eab27d911b515.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Physiological and agro-morphological response of potato to drought stress and hormone applicationپاسخ فیزیولوژیکی و زراعی- مورفولوژیکی سیبزمینی به تنش کمآبی و کاربرد تنظیمکنندههای رشد47611033710.22034/jppb.2019.10337ENAtefehPourasadollahiPhD student of Crop Physiology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.AdelSiosemardehDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.FarzadHosseinpanahiDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.YusefSohrabiDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.Journal Article20181105Potato is considered as a drought sensitive plant. To study the effect of drought stress and hormone on agro-morphological and physiological traits of potato, an experiment was carried out as split plot design based on randomized complete blocks with three replications in 2015 and 2016. The irrigation levels were control (well-watered), mild stress, severe stress and extreme stress, which were arranged in main plots. The second factor included four spaying treatments that were arranged in sub-plots. The foliar applications were as follows: no foliar application (control), gibberellic acid, epibrassinolide and acetyl salicylic acid. Results showed that drought stress and hormones had significant effect on most of the agro-morphological and physiological traits of potato. Leaf dry weight, shoot dry weight, tuber dry weight, plant height, number of stolons, number of tubers, leaf area index (LAI), relative water content (RWC), net photosynthesis rate, transpiration rate, intercellular CO<sub>2</sub> concentration and stomatal conductance decreased, while amount of water saturation deficit (WSD) increased by the drought stress. It seems that the negative impact of drought stress on physiological traits, such as RWC, adversely affected the agro-morphological traits of potato. Except for chlorophyll index, hormones significantly affected agro-morphological and physiological traits of the potato plants. Epibrassinolide improved RWC, WUE, intercellular CO<sub>2</sub> concentration, tuber dry matter, plant height, number of stolons, leaf dry weight, shoot dry weight and tuber dry weight, while application of gibberellic acid had better effects on LAI, WSD, transpiration rate and number of tubers as compared to epibrassinolide. In fact, these hormones mitigated the negative effects of drought stress in potato.<br /> https://breeding.tabrizu.ac.ir/article_10337_ed8341aaa494065dcb629e161d3a99b1.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601The effects of methanol and ethanol foliar application under salinity stress on some physiological characteristics of Pelargonium graveolens L.تاثیرمحلول پاشی با اتانول و متانول بر برخی ویژگی های فیزیولوژیک شعمدانی عطری
تحت تنش شوری63731036810.22034/jppb.2019.10368ENLamiaVojodi MehrabaniDepartment of Agronomy, Azarbaijan Shahid Madani University, Tabriz, IranJournal Article20181126To study the effects of methanol and ethanol foliar application (0, 10 and 20 % v/v) and NaCl salinity stress (0, 75 and 150 mM) on yield and some physiological traits of geranium (<em>Pelargonium graveolens</em> L.), a factorial experiment was conducted based on completely randomized design with three replications. Salinity showed significant effect on all characteristics, except root dry weight, chlorophyll b and Fe content. Effect of foliar application of methanol and ethanol was significant on proline, protein, chlorophyll a, essential oil, Fe and K content, root dry weight and IC<sub>50</sub>. The results also revealed the significant interaction of salinity by foliar application of alcohol in relation to the chlorophyll a and protein content. The greatest protein and chlorophyll a contents were recorded by the NaCl<sub>0</sub> + methanol<sub>20% </sub>treatment, which was significantly different from the corresponding control. Dry weight of aerial parts, K/Na ratio, essential oil, K, P, Fe and Zn contents were negatively affected by the salinity stress. With increasing salinity stress the amounts of malondialdehyde and H<sub>2</sub>O<sub>2</sub> were elevated. Among alcohol treatments, methanol foliar application was more effective than ethanol. Methanol had better effect on IC<sub>50</sub>, root dry weight, Fe, K and essential oil content, while ethanol<sub>20%</sub> increased the proline content significantly as compared to the methanol and control treatments. Overall, the results indicated that foliar application of methanol ameliorated the negative effects of salinity in geranium, when averaged over the salinity levels under study.https://breeding.tabrizu.ac.ir/article_10368_7d8acb62dbd8518e2c677ee4e9f94304.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditionsتأثیر تنظیم کننده های رشد گیاه، منبع کربوهیدرات و غلظت آن بر ریزازدیادی و سایر خصوصیات فیزیولوژیکی انگور (Vitis vinifera L. cv. Shahroudi) تحت شرایط درون شیشه ای75821037810.22034/jppb.2019.10378ENAliRezaTarinejadDepartment of Biotechnology, Faculty of Agriculture, Shahid Madani University, Tabriz, IranSalehAmiriAcademic Staff Member of Agricultural Biotechnology Research Institute of Iran (ABRII), Northwest and West Region, Tabriz, Iran.Journal Article20180415The grapevine (<em>Vitis vinifera</em> L.) is a native of central Asia and has been propagated by various methods, including <em>in vitro </em>propagation. Present research was conducted to study the effect of plant growth regulators (BAP, IBA), carbon sources (sucrose, glucose, fructose, table sugar) and their concentrations (30, 60 and 90 g/l) on the proliferation, root induction, protein, chlorophyll and carotenoid contents of the grape (<em>Vitis</em><em> vinifera L</em>. cv. Shahroudi) in <em>in vitro</em> conditions based on a completely randomized design with three replications, using nodal explants. The result showed that the highest rate of proliferation (3.04 shoots per explant) occurred at MS medium, containing 1.5 mg/l BAP plus constant amounts of GA3 (0.3 mg/l) and IBA (0.1 mg/l). The highest rate of root induction (88.88%) was obtained at 1/2 MS medium at 0.5 mg/l IBA. Type and concentration of carbon source had a significant effect on some of the measured characteristics. The highest plantlet height was obtained in MS medium supplemented with 30 g/1 table sugar and sucrose. Also, the highest number of shoots per explant (3.5 shoots) belonged to the use of 30 g/l sucrose in the culture medium, followed by 30 g/l table sugar (3.23 shoots). The highest value of chlorophyll <em>a</em> was observed for 90 g/l glucose, followed by 30 g/l sucrose and 60 g/l table sugar. The highest chlorophyll <em>b </em>contentwas obtained for 60 g/l table sugar, followed by 30 g/l table sugar and sucrose. In general, with regard to most of the characteristics under study, it could be inferred that the propagation efficiency of 30 g/1 sucrose or table sugar was better than other carbon sources. Considering economic reasons and time, these treatments can be recommended for the commercial micropropagation of the Shahroudi cultivar of grapevine, instead of traditional methods of propagation.https://breeding.tabrizu.ac.ir/article_10378_ec1e4b8330c7950979610aa41a8204b4.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Proteomics analysis of canola seeds to identify differentially expressed proteins under salt stressتجزیه پروتئوم بذور کلزا برای شناسائی پروتئینهای تغییر بیان یافته تحت تنش شوری83951038610.22034/jppb.2019.10386ENSamanehEyvazlouDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.AliBandehaghDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.MajidNorouziDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.MahmoudToorchiDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.0000-0001-7123-9914RezaShokri GhareloDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.Journal Article20171002Seeds are an important part of the canola plant, and similar to other parts are affected by salt stress. Understanding the underlying mechanisms that take place in seeds of canola under salt stress is essential from the perspective of improving quality. In this study, we attempted to identify differentially expressed proteins of canola seeds in the Hyola308 cultivar under 350 mM NaCl using two dimensional (2D) gel electrophoresis. Effect of salt stress was significant on 100-seed weight and number of seeds per plant (p≤ 0.01), and it changed the proteome of the seeds. From a total of 548 reproducible protein spots, 28 protein spots showed significant changes in abundance, of which seven spots showed downregulation. The “Gene Ontology” analysis classified differentially expressed proteins into six biological processes: oxidation-reduction (28.5%), response to abiotic stress (28.5%), response to hormones (21.4%), catabolic process (21.4%), nucleoside diphosphate phosphorylation (17.8%) and glycolytic process (14.28%). In conclusion, salt stress induced canola seeds to upregulate proteins that mostly involved in the antioxidant activity and the proteins with nutrient reservoir activity.https://breeding.tabrizu.ac.ir/article_10386_9b22ac5143b3872d7a0e584df84eeb75.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Contamination control in Iranian seedless barberry micropropagationکنترل آلودگی در ریزازدیادی زرشک بیدانه ایرانی971101038710.22034/jppb.2019.10387ENRahimNazary Moghaddam AghayehDepartment of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.BahramAbedyDepartment of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.AhmadBalandariKhorasan Science and Technology Park (KSTP), Mashhad, Iran.LeilaSamieiDepartment of Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.AliTehranifarDepartment of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.Journal Article20190415Iranian seedless barberry is a species with high degree of contamination in <em>in vitro</em> culture. The current study was aimed to investigate the effects of mercuric chloride (HgCl<sub>2</sub>), sodium hypochlorite (NaOCl) + HgCl<sub>2</sub>, HgCl<sub>2</sub> + 8-hydroxyquinoline, cefotaxime + streptomycin, HgCl<sub>2</sub> + shoot type, HgCl<sub>2</sub> + explant position and HgCl<sub>2</sub> + shoot color on the reduction of contamination. The results showed that the combined use of NaOCl and HgCl<sub>2</sub> was more effective than their single use, but the contamination rate was relatively high. Interestingly, the results showed that explants sterilized with 100 mg/L HgCl<sub>2</sub> and cultured in the medium containing 150 mg/L 8-hydroxyquinoline exhibited the lowest fungal contamination (15%), while those treated with 50 mg/L HgCl<sub>2</sub> and cultured in the 8-hydroxyquinoline-free medium showed the highest fungal contamination (96%). Furthermore, treatment of 75 mg/Lcefotaxime + 70 mg/L streptomycin exhibited no bacterial contamination compared to the control with 58% bacterial contamination. Explants prepared from suckers and treated with 150 mg/L HgCl<sub>2 </sub>showed the lowest rates of fungal (16%) and bacterial (13%) contamination. The results also showed that explants collected from upper position had the lowest rates of fungal (20.87%) and bacterial (18.4%) infection. Explants taken from pinkish-colored shoots showed surprisingly the lowest rates of fungal contamination (13.33%), bacterial contamination (10%) and bud mortality (36.47%). In general, choice of explants from upper position of pinkish-colored suckers, sterilization of them with 100 mg/L HgCl<sub>2 </sub>for 5 min and their culture in medium containing 150 mg/L 8-hydroxyquinoline can be recommended for reducing the contamination.https://breeding.tabrizu.ac.ir/article_10387_906404423f3502f8910d3e601ef5e7fe.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Induced genes expression pattern in response to drought stress at seedling stage of wheatالگوی بیان ژنهای القایی در پاسخ به تنش خشکی در مرحله گیاهچه گندم1111281038810.22034/jppb.2019.10388ENBorzoKazeraniDepartment of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.0000-0001-8923-015XSaeidNavabpourDepartment of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.0000-0002-4710-4750Journal Article20190218Molecular studies have shown that several genes with various functions are induced by drought stress and various transcription factors are involved in the regulation of the stress-inducible genes. To evaluate the effects of drought stress on gene expression, CRTBF2, DREB6 and DBF transcription factors as well as the expression pattern of <em>Acyl</em>, <em>SuT5</em> and <em>SuT4</em> genes were studied using qRT-PCR. The experiment was carried out as factorial based on completely randomized design. One factor included drought stress levels of -1, -2, -4 and -6 bar imposed by polyethylene glycol plus a control. The second factor consisted of four wheat cultivars as follows: Qaboos and Koohdasht (as drought tolerant), and Ehsan and Morvarid (as drought sensitive). Assessing the seedling morphological traits, such as germination percentage, plumule length and radicle length showed that with an increase in drought stress, the tolerant cultivars of Qaboos and Koohdasht showed lower decrease for these traits compared to the sensitive cultivars of Ehsan and Morvarid. The results of evaluating the gene expressions demonstrated that the <em>DREB6</em> gene expression was higher in the tolerant cultivars of Qaboos and Koohdasht as compared to the sensitive cultivars of Ehsan and Morvarid, indicating the possible role of the product of this gene, as a transcription factor, in inducing promoters of drought tolerance genes and transcribing such genes. However, the <em>CRTBF2</em> and <em>DBF</em> genes acted as repressor transcription factors in expressing the genes involved in the drought stress tolerance in the cultivars under study. The expressions of the <em>Acyl</em> and <em>SuT5</em> genes were greater in the tolerant cultivars compared to the sensitive cultivars. The <em>SuT4</em> gene expression was lower in both tolerant and sensitive cultivars than the expression of this gene in the corresponding control treatment. Therefore, it seems that the <em>Acyl</em> and <em>SuT5</em> genes, through lipid decomposition and carbohydrate transport pathways, respectively, increased the tolerance of Qaboos and Koohdasht cultivars in this study.https://breeding.tabrizu.ac.ir/article_10388_d7e9ce86fab86f7a8a0d0b3e6a920f0b.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601In vitro regeneration of periwinkle (Catharanthus roseus L.) and fidelity analysis of regenerated plants with ISSR Markersباززایی درون شیشه ای گیاه پروانش (.Catharanthus roseus L) و بررسی پایداری ژنتیک گیاهچه های حاصل از کشت بافت با استفاده از نشانگرهای ISSR1291351038910.22034/jppb.2019.10389ENSalehAmiriDepartment of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.RezaFotovatDepartment of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.0000-0002-0515-7969Ali RezaTarinejadDepartment of Plant Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.BahmanPanahiDepartment of Genomics, Northwest and West Region Branch, Agricultural Biotechnology Research Institute, Tabriz, Iran.Seyed AbolghasemMohammadiDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.Journal Article20190403<em>Catharanthus roseus</em> is an important multipurpose medicinal plant. In this study, <em>in vitro</em> proliferation and root induction of periwinkle were optimized and regenerated plants were subsequently surveyed for genetic homogeneity using the inter simple sequence repeat (ISSR) markers. Shoot tips and nodal segments were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations of benzylamino-purine (BAP), gibberellic acid (GA3), and indol-3-butyric acid (IBA) hormones. ISSR profiling of regenerated plants as well as the mother plant were surveyed with five primers. The highest establishment rate (80.67%) was obtained in the MS medium containing 1.0 mg L<sup>-1</sup> GA3 and 1.0 mg L<sup>-1</sup> BAP. Highest proliferation rate (5.20 shoots/explant) and average shoot length (6.30 cm) were observed in 1.5 mg L<sup>-1</sup> BAP + 0.5 mg L<sup>-1</sup> IBA. Moreover, the best rooting response (85.30%) was observed on half strength MS containing 1.0 mg L<sup>-1</sup> IBA. Genetic fidelity analysis using ISSR markers showed the monomorphic banding pattern of the micro-propagated plants and the mother plant, which highlighted their genetic uniformity. This implies that periwinkle micropropagation through shoot tip is the most reliable method for true-to-type production of <em>C. roseus</em> in a large scale<em>.</em>https://breeding.tabrizu.ac.ir/article_10389_b34b51c90791d38fc9fc19b47de8f73f.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Morphological and molecular study of sex determination in Cannabis sativa L.مطالعه مورفولوژیکی و مولکولی تعیین جنسیت در شاهدانه1371461039710.22034/jppb.2019.10397ENMaryamRahimiMSc student, Department of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.MajidShokrpourDepartment of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.0000-0001-9377-554XSeyed AlirezaSalamiDepartment of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.Mohammad RezaTaheriDepartment of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.Journal Article20180603<em>Cannabis sativa</em> L. is an important plant with various uses in pharmaceutical and paper production industries. Due to the higher priority of female and male <em>Cannabis</em> plants for pharmaceutical uses and fibre industry, respectively, reduction of the quality of products after pollination, and also for detection of better genotypes before pollination for breeding purposes, early determination of sex in <em>Cannabis</em> is one of the major concerns of researchers and farmers. Seeds of 26 accessions from different regions of Iran along with one accession from Afghanistan were planted in the field based on randomized complete block design with 10 replications. Five female and five male plants were sampled from each accession for molecular and morphological analyses. Thirteen ISSR primers and two SCAR markers were used. Also, morphological differences, which possibly related to sex were measured in the <em>Cannabis</em> populations under study. The results showed significant differences among the accessions within female and male plants for plant height, number of days to flowering, days after full bloom and height of the first flowering node. Male plants were generally, but not always, taller than female plants and had a shorter life cycle. The highest expected heterozygosity was found in the ISSR3 primer and the lowest in the UBC825 primer. In total, 143 polymorphic bands and one polymorphic band were amplified using ISSR and SCAR primers, respectively. Out of 143 polymorphic ISSR bands, only 10 markers had significant relations with the gender of this plant. MADC6 SCAR marker was monomorph across the accessions. On the other hand, MADC5 was a polymorphic marker and showed a significant relation with gender. These markers, especially MADC5, have the potential to be used in sex determination of <em>C. sativa</em>.https://breeding.tabrizu.ac.ir/article_10397_4a11b7650781432a7f1c816c2541e336.pdfUniversity of TabrizJournal of Plant Physiology and Breeding2008-51689120190601Salinity stress responsive leaf proteins in alfalfa (Medicago sativa L.)پروتئین های پاسخ دهنده به تنش شوری در یونجه (.Medicago sativa L)1471571040310.22034/jppb.2019.10403ENZahraDehghanianDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.AliBandehaghDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.AdelDabbagh Mohammadi NasabDepartment of Plant Ecophysiology, Faculty of Agriculture University of Tabriz, Tabriz, Iran.Journal Article20181228Salinity stress is one of the most harmful abiotic stresses that affect agronomical, physiological and biochemical processes in crop plants. In order to evaluate the effect of salinity stress on alfalfa (Bami ecotype) leaf proteins based on two-dimensional gel electrophoresis, an experiment was conducted with two salinity treatments (0 and 200 mM NaCl) and six replications under hydroponic cultural system. Salinity stress decreased fresh and dry weight of alfalfa about 24.5 and 39.5 percent, respectively. Proteome analysis was carried out on the leaf tissue using three replications. Fourteen repeatable protein spots had significant change in expression under salt stress. Probabilistic identification of proteins from the data bank was performed by isoelectric point and molecular weight. Nine proteins showed significant up-regulation and five proteins had down regulation. Candidate proteins were among the proteins involved in the defense system, regulation, metabolic pathways, nitrogen fixation and canalization. Higher abundance of trehalose-phosphate phosphatase 2, involved in regulation, and also uracil phosphoribosyl transferase lastic and β-hydroxyisobutyryl-CoA hydrolase 1, involved in the energy metabolism, demonstrated the important function of these proteins under salinity stress. It seems that the alfalfa plant manages to reduce the adverse effects of salinity through the candidate proteins identified in this investigation. <br /> https://breeding.tabrizu.ac.ir/article_10403_4435988dea28b8d845d62b1622330ba8.pdf