University of Tabriz Journal of Plant Physiology and Breeding 2008-5168 14 1 2024 06 01 Influence of Arbuscular mycorrhiza fungi and Bradyrhizobium japonicum on the fatty acids profile of soybean (Glycine max L.) under drought stress conditions 125 146 19118 10.22034/jppb.2024.59960.1326 EN Naser Samsami Department of Agronomy, Gonbad Kavous University, Gonbad Kavous, Iran Ali Nakhzari Moghaddam Department of Agronomy, Gonbad Kavous University, Gonbad Kavous, Iran Ali Rahemi Karizaki Department of Agronomy, Gonbad Kavous University, Gonbad Kavous, Iran Esmaeil Gholinezhad Department of Agricultural Sciences, Payame Noor University, Tehran, Iran Journal Article 2024 01 06 <strong>Objective</strong>: Water deficiency changes the composition of soybean fatty acids. Arbuscular mycorrhiza fungi and <em>Bradyrhizobium </em>play a vital role in the improvement of soybean protein and oil. Therefore, this study aimed to evaluate the effects of different drought levels and inoculation/non-inoculation with mycorrhiza fungi and <em>Bradyrhizobium </em>on the soybean oil, protein percentage, and fatty acid composition.<br /><strong>Methods</strong>: This experiment was conducted a split-plot factorial layout based on a randomized complete block design with three replications in 2017. The main factor was irrigation after 70, 110, and 150 mm of evaporation from a class A evaporation pan. Subplots included mycorrhiza fungal inoculations with <em>Funneliformis mosseae, Rhizophagus intraradices,</em> and without fungus, and bacterium inoculation at two levels<em> (Bradyrhizobium</em> <em>japonicum</em> and without bacteria).<br /><strong>Results</strong>: Drought stress significantly reduced the oil percentage. The highest oil content (21.94%) was observed in <em>F. mosseae </em>mycorrhiza inoculation. The highest amount of unsaturated fatty acid (74.75%) was observed after inoculation with <em>R. intraradices</em> and bacteria under full irrigation conditions. Inoculation with mycorrhiza fungi and bacteria increased the unsaturated fatty acids in some cases.<br /><strong>Conclusion</strong>: In conclusion, to improve the oil and protein percent, enhance unsaturated fatty acids, and reduce saturated fatty acids in the soybean, the application of mycorrhiza fungi and inoculation with <em>B. japonicum</em> may be beneficial.<br />  https://breeding.tabrizu.ac.ir/article_19118_be6030715b47f688ca384ace62da69aa.pdf