University of Tabriz Journal of Plant Physiology and Breeding 2008-5168 12 1 2022 06 30 Effects of selenium nanoparticles and ancymidol on physiological responses of Stevia rebaudiana Bertoni colonized by Piriformospora indica اثرات کاربرد نانوذرات سلنیوم و آنسیمیدول بر پاسخ های فیزیولوژیکی گیاه Stevia rebaudiana Bertoni تلقیح شده با قارچ Piriformospora indica 67 92 16039 10.22034/jppb.2022.16039 EN Roya Karamian Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran Masoumeh Ahmadi Khoei Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran Journal Article 2021 12 17 The South American plant, <em>Stevia rebaudiana</em> Bertoni, is a good source of steviol glycosides, antioxidants, all of the essential amino acids, and other important nutritional compounds. The present study was conducted to investigate the effects of 5 and 10 mg L^-1 concentrations of selenium nanoparticles (SeNPs) and 50 mg L^-1ancymidol (ANC) on physiological and biochemical characteristics of <em>S. rebaudiana</em> colonized by the root endophytic fungus <em>Piriformospora</em> <em>indica</em> at vegetative and initial flowering stages. Results indicated that ANC decreased root colonization rate and antioxidant enzyme activities, but increased the content of H₂O₂ (vegetative stage), malondialdehyde (initial flowering stage), and phosphorus uptake (initial flowering stage( significantly and had no effect on total carbohydrate content. The adverse effects of ANC reduced after <em>P. indica</em> colonization and somewhat with SeNPs application. Our results suggest that the <em>P.</em> <em>indica</em> colonization and SeNPs application can alter the equilibrium between the production of free radicals and enzymatic defence reactions in <em>S.</em> <em>rebaudiana</em> by enhancing the scavenging capacity of free radicals and by decreasing membrane lipid peroxidation during both vegetative and initial flowering stages. Moreover, the effects of ANC, SeNPs, and <em>P.</em> <em>indica</em> on the measured characteristics at the vegetative stage were higher than those observed at the initial flowering stage. https://breeding.tabrizu.ac.ir/article_16039_71f261cdcf81af53f30e753aa4f57a89.pdf