Genetic Variability and QTL Mapping for Seed Germination Characters under Water Stress in Sunflower

Contributors

Abstract

The objective of this research was to study the genetic control of germination variables under normal and water deficit conditions in sunflower. A population of 100 recombinant inbred lines obtained from a cross between PAC2 and RHA266 was used in the present study. The experiment was performed as a randomized complete block design with three replications. Germination variables were time to starting germination (TSG), time to maximum germination (TMG), time to 50% germination (T50%G) and percentage of seed germination (PSG). The putative causes of variation in germination, oil content (OC) and 1000 grain weight (1000GW) were also measured. Positive correlations were obtained between different times to germination for normal and stress conditions. Positive correlations were observed between PSG and OC, as well as between 1000 GW and the different times to germination. Several non-specific QTLs to water deficit were detected for germination parameters. “HA1837”, “SSL27“and “ORS671_1” were the SSR markers associated with QTLs detected for germination characters independent of germination environments. The most important specific QTL was associated with “ORS677” SSR marker in the water stress condition, explaining 24% of the phenotypic variance for PSG.    
 

Keywords

Article Title [Persian]

تنوع ژنتیکی و مکان‌یابی QTL برای صفات جوانه زنی در آفتابگردان تحت شرایط تنش کم‌آبی

Abstract [Persian]

این تحقیق روی کنترل ژنتیکی صفات جوانه زنی در شرایط نرمال و تنش کم­آبی در آفتابگردان انجام گرفت. جمعیتی شامل 100 لاین اینبرد حاصل از تلاقی RHA266 ×PAC2  مورد استفاده واقع شد. آزمایش به صورت طرح بلوک کاملا تصادفی در سه تکرار در هر یک از شرایط انجام شد. متغیر­های مورد بررسی شامل زمان­های تا جوانه زنی و درصد جوانه زنی بودند. میزان روغن بذر و وزن 1000 دانه نیز اندازه گیری شد. همبستگی مثبت بین تمامی زمان­های تا جوانه زنی دیده شد. همچنین همبستگی مثبت بین درصد جوانه زنی و میزان روغن بذر و وزن 1000 دانه وجود داشت. تعدادی QTL غیراختصاصی برای شرایط محیطی مختلف در مورد صفات جوانه زنی به دست آمدند. نشانگر­های “ORS671_1”، “HA1837” و “SSL27“ برای صفات جوانه زنی، که مستقل از شرایط محیطی بودند، شناسایی شدند. QTL مهمی نیز برای درصد جوانه زنی در شرایط تنش کم­آبی که با نشانگر “ORS 677” پیوسته بوده و 24% از تغییرات این صفت را کنترل می­کرد، شناسایی شد.
 

Keywords [Persian]

  • تنش خشکی
  • زمان­های تا جوانه زنی
  • نشانگر
  • QTL

References

Ahmad S, 2001. Environmental effect on seed characteristics of sunflower (Helianthus annuus L.). Journal of Agronomy and Crop Science 187: 213-216.
Al-Chaarani, GR, Gentzbittel L, Wedzony M and Sarrafi A, 2005. Identification of QTLs for germination and seedling development in sunflower (Helianthus annuus L.). Plant Science 169: 221-227.
Basten CJ, Weir BS and Zeng ZB, 2001. QTL Cartographer, Version 1.15. Department of Statistics, North Carolina State University, Raleigh, NC, USA.
Bettey M, Finch-Savage W, King GJ  and Lynn JR, 2000. Quantitative genetic analysis of seed vigour and pre-emergence seedling growth traits in Brassica oleracea. New Phytologist 148: 277-286.
Bradford KJ, 1995. Water relations in seed germination. In: Kigel J and Galili G (Eds). Seed Development and Germination, Pp. 351-396. Marcel Dekker, New York.
Cook RE, 1979. Patterns of juvenile morbidity and recruitment in plants. In: Solbrig OT, Jain S, Johnson GB and Raven PH (Eds). Topics in Plant Population Biology.Pp. 207-301. Columbia University Press, Los Angeles.
  Ebrahimi A, Maury P, Berger M, Poormohammad Kiani S, Nabipour A, Shariati F, Grieu P and Sarrafi A, 2008. QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes. Genome 51: 599–615.
Ecker R, Barzilay A and Osherenko E, 1994. The genetic relations between length of time to germination and seed dormancy in lisianthus (Eustomia grandiflorum). Euphytica 80: 125-128.
Evans CE and Etherington JR, 1990. The effect of soil water potential on seed germination of some British plants. New Phytologist 115: 539-548.
Feussner I, Hause B, Nellen A, Wasternack C and Kindl H, 1996. Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms. Planta 198: 288-293.
Foolad MR, 1999. Genetics of salt tolerance and cold tolerance in tomato: Quantitative analysis and QTL mapping. Plant Biotechnology 16: 55-64.
Foolad MR, Chen FQ and Lin GY, 1998. RFLP mapping of QTLs conferring salt tolerance during germination in an interspecific cross of tomato. Theoretical and Applied Genetics 97: 1133-1144.
Foolad MR and Jones RA, 1991. Genetic analysis of salt tolerance during germination in Lycopersicon. Theoretical and Applied Genetics 81: 321-326.
Foolad, MR and Lin GY, 1999. Relationships between cold and salt-tolerance during seed germination in tomato: Germplasm evaluation. Plant Breeding 118: 45-48.
Foolad MR, Lin GY and Chen FQ, 1999. Comparison of QTLs for seed germination under non-stress, cold stress and salt stress in tomato. Plant Breeding 118: 167-173.
Foolad MR, Zhang LP and Subbiah P, 2003.Genetics of drought tolerance during seed germination in tomato: Inheritance and QTL mapping.Genome 46: 536-545.
Heydecker W, Higgins J and Turner YJ, 1975. Invigoration of seeds. Seed Science and Technology 3: 881-888.
Jahromi PH, 1996. Genetic studies on salt tolerance in barley (Hordeum vulgare L.), PhD Thesis, The University of Reading, UK.
Jones RA, 1986. High salt-tolerance potential in Lycopersicon species during germination. Euphytica 35: 576-582.
Kassem AM, Meksem K, Wood AJ and Lightfoot DA, 2007. A microsatellite map developed from late maturity germplasm 'Essex' by 'Forrest' detects four QTL for soybean seed yield expected from early maturing germplasm. Reviews in Biology and Biotechnology 6: 11-19.
Kearsey MJ, 1998. The principles of QTL analysis (a minimal mathematics approach). Journal of Experimental Botany 49: 1619-1623.
Lenzi A, Fambrini M, Barotti S, Pugliesi C and Vernieri P, 1995. Seed germination and seedling growth in a wilty mutant of sunflower (Helianthus annuus L.): effect of abscisic acid and osmotic potential. Environmental and Experimental Botany 35: 427-434.
Lin Y and Huang AHC, 1983. Lipase in lipid bodies of cotyledons of rape and mustard seedlings. Archives of Biochemistry and Biophysics 225: 360-369.
May C, Preisig-Müller R, Höne M, Gnau P and Kindl H, 1998. A phospholipase A2 is transiently synthesized during seed germination and localized to lipid bodies. Biochimica et Biophysica Acta- Lipids and Lipid Metabolism 1393: 267-276.
Poormohammad Kiani S, Talia P, Maury P, Grieu P, Heinz R, Perrault A, Nishinakamasu V, Hopp E, Gentzbittel  L, Paniego N and Sarrafi A, 2007. Genetic analysis of plant water status and osmotic adjustment in recombinant inbred lines of sunflower under two water treatments. Plant Science 172: 773-787.
Roberts EH and Ellis RH, 1989. Water and seed survival. Annals of Botany 63: 39-52.
Sadeghipour HR and Bhatla SC, 2002. Differential sensitivity of oleosins to proteolysis during oil body mobilization in sunflower seedlings. Plant and Cell Physiology 43: 1117-1126.
Saleki R, Young PG and Lefebvre DD, 1993. Mutants of Arabidopsis thaliana capable of germination under saline conditions. Plant Physiology 101: 839-845.
Singh CB, Dalai MA and Singh SP, 1978. Genetic analysis of field germination in soybean. Theoretical and Applied Genetics 52: 165-169.
Terry LT, 1993. Gene expression during plant embryogenesis and germination. The Plant Cell 5: 1401-1410.
Torres MG, Frutos S and Duran JM, 1991. Sunflower seed deterioration from exposure to UV-C radiation. Environmental and Experimental Botany 31: 201-207.
Turhan H and Baser I, 2004.  In vitro and In vivo water stress in sunflower (Helianthus annuus L.). Helia 27: 227-236.
Ujjinaiah US, Shauthamallaiah NR and Murali WM, 1989. Effect of different row spacing and N and P2O5 fertilizer levels on growth, yield, yield components and quality of seed in sunflower. Mysore Journal of Agricultural Sciiences 23: 146-850.
Wang SM and Huang AHC, 1987. Biosynthesis of lipase in the scutellum of  maize Kernel. The Journal of Biological Chemistry 262: 2270-2274.
Wang S, Basten CJ and Zeng ZB 2005. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, USA.         
Zeng ZB, 1993. Theoretical basis of separation of multiple linked gene effects on mapping quantitative trait loci. Proceedings of the National Academy of Sciences, USA. 90: 10972–10976.
Zeng ZB, 1994. Precision mapping of quantitative trait loci. Genetics 136: 1457–1468.

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